Rushville Republican

Agriculture

March 7, 2014

Gene identified by Purdue scientists may ease the genetic modification of plants

(Continued)

A paper detailing the team’s results was published in Science Signaling and is available online. In addition to Gelvin, co-authors include Rebecca Doerge, the Trent and Judith Anderson Distinguished Professor of Statistics; former postdoctoral research fellows and research associates graduate students Nagesh Sardesai, Huabang Chen and HoChul Yi; associate research scientist Lan-Ying Lee; visiting scholar Alexandra Stirnberg; former graduate student Gayla R. Olbricht; and undergraduate students Jacob Jeffries and Kia Xiong.

The team conducted genetic screens of Arabidopsis mutants hyper-susceptible to Agrobacterium infection to identify genes responsible for susceptibility. Through these screens the team discovered that a mutation in the gene MTF1 affected susceptibility to infection and genetic transformation. Plants in which MTF1 is suppressed were more susceptible to transformation.

The team also found that cytokinin, a plant hormone secreted by Agrobacteria, triggers a cascade of molecular events that lead to the suppression of MTFI and the activation of another gene that may aid the infection process. The gene that is activated encodes a protein that sits on the surface of the cell and may allow Agrobacterium to bind better to the plant cell, Gelvin said.

“We uncovered an entire molecular signal transduction pathway that reveals how the secreted cytokinins suppress the expression of the gene MTF1, which in turn increases the expression of the gene AT14A and a protein that makes a plant more susceptible to infection byAgrobacterium,” he said. “The end result is a plant that is much more susceptible to genetic transformation. Perhaps reducing the expression of MTF1 in other plants will have the same effect.”

MTF1 is important for normal plant development, so it cannot be completely knocked out or eliminated without killing the plant, but there are ways to reduce its expression, Gelvin said.

The team is working on developing technology to translate their findings into a tool that can reduce the expression of MTF1 and genetically transform a plant in one step, he said.

Text Only
Agriculture
  • USDA reminds farmers of 2014 Farm Bill Conservation Compliance changes WASHINGTON - Agriculture Secretary Tom Vilsack today reminded producers that changes mandated through the 2014 Farm Bill require them to have on file a Highly Erodible Land Conservation and Wetland Conservation Certification (AD-1026). The Farm Bill

    July 25, 2014

  • Extension farm tour to feature organic vegetable production WEST LAFAYETTE - The organic vegetable production and fertility management practices at an Ohio farm will be showcased in a Purdue Extension tour of the operation near Cincinnati.The tour will be from 9 a.m. to noon Aug. 5 at EcOhio Farm, 2210 S. Mas

    July 18, 2014

  • ag-rv071814-soybean variety pic Gene discovery could lead to better soybean varieties for northern United States WEST LAFAYETTE – Researchers from Purdue University and the University of Nebraska-Lincoln have discovered a soybean gene whose mutation affects plant stem growth, a finding that could lead to the development of improved soybean cultivars for the nor

    July 18, 2014 1 Photo

  • U.S. farmers plant record soybean crop, less corn DES MOINES, Iowa (AP) — The nation’s farmers planted the largest soybean crop on record this year by devoting millions of acres of land to the crop that had been used for growing corn, the U.S. Department of Agriculture said Monday.Farmers planted 84

    July 3, 2014

  • Manure Management Field Day presents new technology NORTH MANCHESTER — The Wabash County Soil & Water Conservation District and the Conservation Cropping Systems Initiative will host a free Manure Management Field Day on July 29th at Brubaker Farms. The half-day program will address application techno

    July 3, 2014

  • Purdue, OSU assisting in ag meeting for Corn Belt farmers WEST LAFAYETTE — Purdue and Ohio State Universities are part of an organization that is sponsoring a meeting this summer to help Corn Belt farmers make their agricultural systems more resilient and sustainable.The Resilient Agriculture Conference Au

    June 27, 2014

  • Farm Credit supports Rushville City FD training efforts RUSHVILLE — Farm Credit Mid-America, an agricultural lending cooperative serving farmers, rural residents and agribusinesses throughout Indiana, Ohio, Kentucky and Tennessee, contributed $500 to the Rushville City Fire Department. The funds were used

    June 27, 2014

  • ag-rv062714-problem weeds pic Latest weed control advances in corn, cotton COLLEGE STATION — The latest strategies in managing problem weeds in corn and cotton were recently showcased at the 2014 Crop Tour at the Texas A&M University field laboratory near College Station that serves as a research and teaching platform for T

    June 27, 2014 1 Photo

  • Farm Credit salutes Rushville FFA graduating seniors RUSHVILLE — Farm Credit Mid-America, an agricultural lending cooperative serving farmers, rural residents and agribusinesses throughout Indiana, Ohio, Kentucky and Tennessee, contributed $500 to support the 2014 Rushville FFA banquet, themed “Legacy

    June 27, 2014

  • Purdue fruit, vegetable food safety course now offered online WEST LAFAYETTE - Purdue Extension is now offering in an online format a course covering food safety practices for fruit and vegetable growers.The course is based on a series of Good Agricultural Practices from A to Z workshops that were given in the

    June 20, 2014